Jose L. Agraz, PhD

PHIP Process

The product is then delivered intravenously for in vivo imaging

HEP Molecule

Target hyperpolarized molecule

Molecule RF Irradiation

The Rh complex dislodges. The spin alignment then transfers from the added p-H₂ atoms to ¹³C in the newly created molecule by irradiating the mix with RF

HEA para-Hydrogen Bonding

HEA hydrogenation using para-Hydrogen

HEA Rh binding

This precursor is preloaded with a Rh catalyst complex that weakens the ¹³C double bond

PHIP Experiment Structure

A metabolite hyperpolarization experiment begins by heating up the sample and filling up the reaction capsule with p-H2 . at the end, the sample is pushed into an external vial

Hyperpolarizer Box

Hyperpolarizer Inside Box

Hyperpolarization Graphical Abstract

The PHIP method concept is based in the chemical addition of p-H₂ to a substrate or precursor molecule (gas or liquid).

Proton MRI Signal

Frequency spectrum of a normal 13C molecule signal. Very small signal amplitude at equilibrium

Hyperpolarization MRI Signal

Frequency spectrum of hyperpolarized 13C molecule. 10,000 times more signal amplitude

Hyperpolarization Reaction Capsule

The mixing of p-H2 and the precursor occurs in a reaction chamber where a catalyst assisted hydrogenation reaction takes place, adding the two p-H2 atoms across the carbon-carbon double bond

Hyperpolarization LabView Software

Blue labels point to the panel’s four different sections: Pinch Valve Indicators, Experiment Timing Setup, TabControl Setup, and Status Bar

Software Structure

With 46 controls and 36 indicators on the SFP, the program is too large for linear programming. Instead, the program is event driven, allowing for more efficient program execution